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Cloning of minute virus of mice cDNAs and preliminary analysis of individual viral proteins expressed in murine cells
Journal article   Peer reviewed

Cloning of minute virus of mice cDNAs and preliminary analysis of individual viral proteins expressed in murine cells

K E Clemens, D R Cerutis, L R Burger, C Q Yang and D J Pintel
Journal of virology, Vol.64(8), pp.3967-3973
08/01/1990
PMCID: PMC249693
PMID: 2164605

Abstract

Animals Capsid - genetics Cell Line Cloning, Molecular - methods DNA, Viral - genetics Gene Expression Genetic Vectors Humans Mammary Tumor Virus, Mouse - genetics Mice Minute Virus of Mice - genetics Parvoviridae - genetics Phosphorylation Transcription Factors - genetics Transcription, Genetic Transfection Viral Core Proteins - genetics Viral Nonstructural Proteins Viral Proteins - analysis Viral Proteins - genetics
cDNAs corresponding to RNA from the autonomous parvovirus minute virus of mice were cloned into constitutive and inducible expression vectors. These clones generate viral NS2, VP1, and VP2 proteins individually. Initial examination of these clones by transient expression analysis and analysis of stably transformed murine cell lines inducibly expressing these constructs indicated that they will be useful tools for characterizing the function of individual minute virus of mice gene products.

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