Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer; Mark D. Reisbig; Nancy D. Hanson

doi:10.1128/JCM.02038-12

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Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Journal article

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Peer reviewed

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes

Chelsie N. Geyer, Mark D. Reisbig and Nancy D. Hanson

Journal of clinical microbiology, Vol.50(11), pp.3722-3725

11/2012

DOI: https://doi.org/10.1128/JCM.02038-12

PMCID: PMC3486237

PMID: 22895035

Abstract

Microbiology (medical)

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

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Title: Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated AmpC β-lactamase genes
Creators: Chelsie N. Geyer (Author) - Creighton University
Mark D. Reisbig (Author) - Department of Anesthesiology
Nancy D. Hanson (Author) - Department of Medical Microbiology and Immunology
Publication Details: Journal of clinical microbiology, Vol.50(11), pp.3722-3725
Series: 50
Number of pages: 4
Identifiers: 991005930282202656
Academic Unit: Anesthesiology; Med. Microbiology & Immunology
Language: English
Resource Type: Journal article