Logo image
Sign in
Back
Direct fluorescent-antibody testing followed by culture for diagnosis of 2009 H1N1 influenza A
Journal article   Open access

Direct fluorescent-antibody testing followed by culture for diagnosis of 2009 H1N1 influenza A

Paul Bakerman, Lilanthi Balasuriya, Ora Fried, David Tellez, Pamela Garcia-Filion and Heidi Dalton
Journal of Clinical Microbiology, Vol.49(10)
2011
DOI: https://doi.org/10.1128/JCM.05255-11

Abstract

Adolescent Adult Antibodies, Viral Antigens, Viral Child Child, Preschool Clinical Laboratory Techniques Fluorescent Antibody Technique, Direct Humans Infant Infant, Newborn Influenza A Virus, H1N1 Subtype Influenza, Human Sensitivity and Specificity Virology Young Adult monoclonal antibody 2009 H1N1 influenza antibody detection article diagnostic accuracy fluorescent antibody technique human hydrolysis Influenza virus A H1N1 medical record pediatric hospital polymerase chain reaction predictive value priority journal real time polymerase chain reaction retrospective study reverse transcription polymerase chain reaction sensitivity and specificity virus culture virus identification virus strain
During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescentantibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibody testing, which missed one-third of infected individuals. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
url
https://doi.org/10.1128/JCM.05255-11View
Published (Version of record) Open

Metrics

1 Record Views

Details

Logo image