Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase

L. H. Wang; Y. P. Tu; X. Y. Yang; Z. C. Tsui; F. Y. Yang

doi:10.1016/0014-5793(96)00534-0

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Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase

Journal article

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Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase

L. H. Wang, Y. P. Tu, X. Y. Yang, Z. C. Tsui and F. Y. Yang

FEBS Letters, Vol.388(2-3), pp.128-130

388

06/17/1996

DOI: https://doi.org/10.1016/0014-5793(96)00534-0

PMID: 8690070

Abstract

Biophysics

Structural Biology

Biochemistry

Molecular Biology

Genetics

Cell Biology

Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography, to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca2+ uptake activity of sarcoplasmic reticulum Ca2+-ATPase incorporated into the proteoliposomes. Conformation changes of Ca2+-ATPase induced by GM3 were also observed by intrinsic fluorescence and circular dichroism measurements.

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Title: Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase
Creators: L. H. Wang (Author) - Dalian Medical University
Y. P. Tu (Author) - CAS - Institute of Biophysics
X. Y. Yang (Author) - Institute of Biophysics
Z. C. Tsui (Author) - Dalian Medical University
F. Y. Yang (Author) - CAS - Institute of Biophysics
Additional links: Wang, P.P.; Dai, J.; Tyan, J.C.-Y.
Publication Details: FEBS Letters, Vol.388(2-3), pp.128-130
Series: 388
Number of pages: 3
Identifiers: 991005930520602656
Academic Unit: Creighton University
Language: English
Resource Type: Journal article

Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+-ATPase

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