Abstract
The purpose of this study was to determine the effects of resistance training prior to and during doxorubicin (DOX) treatment on antioxidant enzyme expression in the primarily type I soleus muscle. Thirty‐six male, Sprague‐Dawley rats were randomly assigned to one of four groups: sedentary+saline (SSS), sedentary+DOX (SSD), resistance training+saline (RRS), and resistance training+DOX (RRD). The resistance training protocol incorporated a raised cage model where food and water were elevated progressively which provided hindlimb loading 10 weeks prior to DOX injection and during the course of DOX treatment. Animals receiving DOX received 3 mg/kg DOX administered i.p. weekly for 4 weeks (12 mg/kg cumulative). Five days following the final DOX injection, solei were excised and Western blotting was performed to quantify superoxide dismutase (SOD)1 and SOD2 expression. No significant main activity or drug effects were observed with SOD1 or SOD2 expression, but a significant activity × drug interaction was observed for SOD1 expression with RRD expressing significantly higher SOD1 than SSD. This interaction, however, was not observed for SOD2. In conclusion, resistance training prior to and during weekly DOX administration promoted an increase in SOD1 expression (the cytosolic form of SOD) suggesting that resistance training may play a role in combatting DOX myotoxicity by enhancing cytosolic antioxidant defenses.
This is from the Experimental Biology 2018 Meeting. There is no full text article associated with this published in The FASEB Journal.