From sequence to chromosome: The tip of the X chromosome of D. melanogaster

Panayiotis V. Benos; Melanie K. Gatt; Michael Ashburner; Lee Murphy; David Harris; Bart Barrell; Conception Ferraz; Sophie Vidal; Christine Brun; Jacques Demailles; Edouard Cadieu; Stephane Dreano; Stéphanie Gloux; Valerie Lelaure; Stephanie Mottier; Francis Galibert; Dana Borkova; Belen Minana; Fotis C. Kafatos; Christos Louis; Inga Sidén-Kiamos; Slava Bolshakov; George Papagiannakis; Lefteris Spanos; Sarah Cox; Encarnación Madueño; Beatriz De Pablos; Juan Modolell; Annette Peter; Petra Schöttler; Meike Werner; Foteini Mourkioti; Nicole Beinert; Gordon Dowe; Ulrich Schäfer; Herbert Jäckle; Alain Bucheton; Deborah M. Callister; Lorna A. Campbell; Areti Darlamitsou; Nadine S. Henderson; Paul J. McMillan; Cathy Salles; Evelyn A. Tait; Phillipe Valenti; Robert D. C. Saunders; David M. Glover

doi:10.1126/science.287.5461.2220

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From sequence to chromosome: The tip of the X chromosome of D. melanogaster

Journal article

Peer reviewed

From sequence to chromosome: The tip of the X chromosome of D. melanogaster

Panayiotis V. Benos, Melanie K. Gatt, Michael Ashburner, Lee Murphy, David Harris, Bart Barrell, Conception Ferraz, Sophie Vidal, Christine Brun, Jacques Demailles, …

Science, Vol.287(5461)

2000

DOI: https://doi.org/10.1126/science.287.5461.2220

Abstract

Animals

Chromosome Banding

Computational Biology

Cosmids

DNA Transposable Elements

DNA, Satellite

Drosophila melanogaster

Genes, Insect

In Situ Hybridization

Repetitive Sequences, Nucleic Acid

Sequence Analysis, DNA

X Chromosome

Drosophila melanogaster

Melanogaster

DNA

base pairing

DNA sequence

Drosophila melanogaster

gene sequence

in situ hybridization

nonhuman

nucleotide sequence

priority journal

review

unindexed sequence

X chromosome

One of the rewards of having a Drosophila melanogaster whole-genome sequence wilt be the potential to understand the molecular bases for structural features of chromosomes that have been a long-standing puzzle. Analysis of 2.6 megabases of sequence from the tip of the X chromosome of Drosophila identifies 273 genes. Cloned DNAs from the characteristic bulbous structure at the tip of the X chromosome in the region of the broad complex display an unusual pattern of in situ hybridization. Sequence analysis revealed that this region comprises 154 kilobases of DNA flanked by 1.2- kilobases of inverted repeats, each composed of a 350 - base pair satellite related element. Thus, some aspects of chromosome structure appear to be revealed directly within the DNA sequence itself.

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Title: From sequence to chromosome: The tip of the X chromosome of D. melanogaster
Creators: Panayiotis V. Benos - European Bioinformatics Institute
Melanie K. Gatt - University of Dundee
Michael Ashburner - University of Cambridge
Lee Murphy - Wellcome Sanger Institute
David Harris - Wellcome Sanger Institute
Bart Barrell - Wellcome Sanger Institute
Conception Ferraz
Sophie Vidal - Institut de Génétique Humaine
Christine Brun - Centre National de la Recherche Scientifique
Jacques Demailles - Institut de Génétique Humaine
Edouard Cadieu - Centre National de la Recherche Scientifique
Stephane Dreano - Centre National de la Recherche Scientifique
Stéphanie Gloux - Centre National de la Recherche Scientifique
Valerie Lelaure - Centre National de la Recherche Scientifique
Stephanie Mottier - Centre National de la Recherche Scientifique
Francis Galibert - Centre National de la Recherche Scientifique
Dana Borkova - European Molecular Biology Laboratory
Belen Minana - European Molecular Biology Laboratory
Fotis C. Kafatos - European Molecular Biology Laboratory
Christos Louis - Foundation for Research and Technology Hellas
Inga Sidén-Kiamos - Foundation for Research and Technology Hellas
Slava Bolshakov - European Molecular Biology Laboratory
George Papagiannakis - Foundation for Research and Technology Hellas
Lefteris Spanos - Foundation for Research and Technology Hellas
Sarah Cox - Foundation for Research and Technology Hellas
Encarnación Madueño - Centro de Biología Molecular Severo Ochoa
Beatriz De Pablos - Centro de Biología Molecular Severo Ochoa
Juan Modolell - Autonomous University of Madrid
Annette Peter - Max Planck Institute for Biophysical Chemistry
Petra Schöttler - Max Planck Institute for Biophysical Chemistry
Meike Werner - Max Planck Institute for Biophysical Chemistry
Foteini Mourkioti - Max Planck Institute for Biophysical Chemistry
Nicole Beinert - Max Planck Institute for Biophysical Chemistry
Gordon Dowe - Max Planck Institute for Biophysical Chemistry
Ulrich Schäfer - Max Planck Institute for Biophysical Chemistry
Herbert Jäckle - Max Planck Institute for Biophysical Chemistry
Alain Bucheton - Institut de Génétique Humaine
Deborah M. Callister - University of Dundee
Lorna A. Campbell - University of Dundee
Areti Darlamitsou - University of Dundee
Nadine S. Henderson - University of Dundee
Paul J. McMillan - University of Dundee
Cathy Salles - University of Dundee
Evelyn A. Tait - University of Dundee
Phillipe Valenti - University of Dundee
Robert D. C. Saunders
David M. Glover - University of Cambridge
Publication Details: Science, Vol.287(5461)
Identifiers: 991006151397102656
Language: English
Resource Type: Journal article

From sequence to chromosome: The tip of the X chromosome of D. melanogaster

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