Abstract
To evaluate the effects of DOX on type I and type II muscle function and DOX accumulation. Male Sprague-Dawley rats received 15 g/kg DOX i.p. (n=27) or an equivalent volume of saline i.p. as a control (CON, n=11). Soleus (SOL) and extensor digitorum longus (EDL) function was assessed ex vivo 1 day (n=9), 3 days (n=10), or 5 days (n=8) post DOX injections. DOX accumulation in skeletal muscle was then assessed using high performance liquid chromatography. No significant SOL or EDL maximal twitch force differences were observed 1 day or 3 days post DOX when compared to CON (p>0.05). However, SOL maximal twitch force 5 days post DOX was significantly lower than CON (19+ or -5 mN vs. 39+ or -3 mN, respectively, p<0.01), and EDL maximal twitch force 5 days post DOX was significantly lower than CON (34+ or -4 mN vs. 76+ or -8 mN, respectively, p<0.01).