Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex

Thomas Chartrand; Rachel Dalley; Jennie Close; Natalia A. Goriounova; Brian R. Lee; Rusty Mann; Jeremy A. Miller; Gabor Molnar; Alice Mukora; Lauren Alfiler; Katherine Baker; Trygve E. Bakken; Jim Berg; Darren Bertagnolli; Thomas Braun; Krissy Brouner; Tamara Casper; Eva Adrienn Csajbok; Nick Dee; Tom Egdorf; Rachel Enstrom; Anna A. Galakhova; Amanda Gary; Emily Gelfand; Jeff Goldy; Kristen Hadley; Tim S. Heistek; DiJon Hill; Nik Jorstad; Lisa Kim; Agnes Katalin Kocsis; Lauren Kruse; Michael Kunst; Gabriela Leon; Brian Long; Matthew Mallory; Medea McGraw; Delissa McMillen; Erica J. Melief; Norbert Mihut; Lindsay Ng; Julie Nyhus; Gáspár Oláh; Attila Ozsvár; Victoria Omstead; Zoltan Peterfi; Alice Pom; Lydia Potekhina; Ramkumar Rajanbabu; Marton Rozsa; Augustin Ruiz; Joanna Sandle; Susan M. Sunkin; Ildiko Szots; Michael Tieu; Martin Toth; Jessica Trinh; Sara Vargas; David Vumbaco; Grace Williams; Julia Wilson; Zizhen Yao; Pal Barzo; Charles Cobbs; Richard G. Ellenbogen; Luke Esposito; Manuel Ferreira; Nathan W. Gouwens; Benjamin Grannan; Ryder P. Gwinn; Jason S. Hauptman; Tim Jarsky; C. Dirk Keene; Andrew L. Ko; Christof Koch; Jeffrey G. Ojemann; Anoop Patel; Jacob Ruzevick; Daniel L. Silbergeld; Kimberly Smith; Staci A. Sorensen; Bosiljka Tasic; Jonathan T. Ting; Jack Waters; Christiaan P.J. de Kock; Huib D. Mansvelder; Gabor Tamas; Hongkui Zeng; Brian Kalmbach; Ed.S. Lein

doi:10.1126/SCIENCE.ADF0805

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Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex

Journal article

Peer reviewed

Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex

Thomas Chartrand, Rachel Dalley, Jennie Close, Natalia A. Goriounova, Brian R. Lee, Rusty Mann, Jeremy A. Miller, Gabor Molnar, Alice Mukora, Lauren Alfiler, …

Science, Vol.382(6667)

2023

DOI: https://doi.org/10.1126/SCIENCE.ADF0805

Abstract

Animals

Axons

Humans

Interneurons

Mice

Neocortex

Pyramidal Cells

Transcriptome

axon

transcriptome

divergence

neurology

rodent

signaling

adult

animal cell

animal tissue

article

axon

cytoarchitecture

dendrite

feedback system

female

human

human tissue

interneuron

male

mouse

neocortex

nonhuman

pyramidal nerve cell

signal transduction

animal

interneuron

physiology

Neocortical layer 1 (L1) is a site of convergence between pyramidal-neuron dendrites and feedback axons where local inhibitory signaling can profoundly shape cortical processing. Evolutionary expansion of human neocortex is marked by distinctive pyramidal neurons with extensive L1 branching, but whether L1 interneurons are similarly diverse is underexplored. Using Patch-seq recordings from human neurosurgical tissue, we identified four transcriptomic subclasses with mouse L1 homologs, along with distinct subtypes and types unmatched in mouse L1. Subclass and subtype comparisons showed stronger transcriptomic differences in human L1 and were correlated with strong morphoelectric variability along dimensions distinct from mouse L1 variability. Accompanied by greater layer thickness and other cytoarchitecture changes, these findings suggest that L1 has diverged in evolution, reflecting the demands of regulating the expanded human neocortical circuit. © 2023 American Association for the Advancement of Science. All rights reserved.

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Title: Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex
Creators: Thomas Chartrand - Allen Institute
Rachel Dalley - Allen Institute
Jennie Close - Allen Institute
Natalia A. Goriounova - Vrije Universiteit Amsterdam
Brian R. Lee - Allen Institute
Rusty Mann - Allen Institute
Jeremy A. Miller - Allen Institute
Gabor Molnar - University of Szeged
Alice Mukora - Allen Institute
Lauren Alfiler - Allen Institute
Katherine Baker - Allen Institute
Trygve E. Bakken - Allen Institute
Jim Berg - Allen Institute
Darren Bertagnolli - Allen Institute for Brain Science
Thomas Braun
Krissy Brouner - Allen Institute
Tamara Casper - Allen Institute
Eva Adrienn Csajbok - University of Szeged
Nick Dee - Allen Institute
Tom Egdorf - Allen Institute
Rachel Enstrom - Allen Institute
Anna A. Galakhova - Vrije Universiteit Amsterdam
Amanda Gary - Allen Institute
Emily Gelfand - Allen Institute
Jeff Goldy - Allen Institute
Kristen Hadley - Allen Institute
Tim S. Heistek - Vrije Universiteit Amsterdam
DiJon Hill - Allen Institute
Nik Jorstad - Allen Institute
Lisa Kim - Allen Institute
Agnes Katalin Kocsis - University of Szeged
Lauren Kruse - Allen Institute
Michael Kunst - Allen Institute
Gabriela Leon - Allen Institute
Brian Long - Allen Institute
Matthew Mallory - Allen Institute
Medea McGraw
Delissa McMillen - Allen Institute
Erica J. Melief - University of Washington
Norbert Mihut - University of Szeged
Lindsay Ng - Allen Institute
Julie Nyhus - Allen Institute
Gáspár Oláh - University of Szeged
Attila Ozsvár - University of Szeged
Victoria Omstead - Allen Institute
Zoltan Peterfi - University of Szeged
Alice Pom
Lydia Potekhina - Allen Institute
Ramkumar Rajanbabu - Allen Institute
Marton Rozsa - University of Szeged
Augustin Ruiz - Allen Institute
Joanna Sandle - University of Szeged
Susan M. Sunkin - Allen Institute for Brain Science
Ildiko Szots - University of Szeged
Michael Tieu - Allen Institute
Martin Toth - University of Szeged
Jessica Trinh - Allen Institute
Sara Vargas - Allen Institute
David Vumbaco - Allen Institute
Grace Williams - Allen Institute
Julia Wilson - Allen Institute for Brain Science
Zizhen Yao - Allen Institute
Pal Barzo - University of Szeged
Charles Cobbs - Swedish Medical Center
Richard G. Ellenbogen - Neurological Surgery
Luke Esposito - Allen Institute
Manuel Ferreira - Neurological Surgery
Nathan W. Gouwens - Allen Institute for Brain Science
Benjamin Grannan - Neurological Surgery
Ryder P. Gwinn - Swedish Medical Center
Jason S. Hauptman - Neurological Surgery
Tim Jarsky - Allen Institute
C. Dirk Keene
Andrew L. Ko - Neurological Surgery
Christof Koch - Allen Institute
Jeffrey G. Ojemann - University of Washington
Anoop Patel - Neurological Surgery
Jacob Ruzevick - Neurological Surgery
Daniel L. Silbergeld - Neurological Surgery
Kimberly Smith - Allen Institute
Staci A. Sorensen - Allen Institute for Brain Science
Bosiljka Tasic - Allen Institute
Jonathan T. Ting - Allen Institute
Jack Waters - Allen Institute
Christiaan P.J. de Kock - Vrije Universiteit Amsterdam
Huib D. Mansvelder
Gabor Tamas - University of Szeged
Hongkui Zeng - Allen Institute
Brian Kalmbach - Allen Institute for Brain Science
Ed.S. Lein - Allen Institute
Publication Details: Science, Vol.382(6667)
Publisher: American Association for the Advancement of Science
Identifiers: 991006161397202656
Language: English
Resource Type: Journal article

Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex

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