Abstract
In order to obtain quantities of CPK individual isoenzymes suitable for use in experiments concerned with disappearance of CPK from the circulation, we developed a preparative procedure for isolation of MM, MB and BB CPK from human myocardium and brain. The procedure utilized ethanol fractionation, batch adsorption on DEAE Sephadex A-50, and column chromatography with the same medium. Each isoenzyme fraction was virtually free from activity attributable to the other isoenzymes demonstrable by polyacrylamide gel electrophoresis, although slight protein contamination was detected by SDS gel electrophoresis. © 1976.