Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex

Brian R. Lee; Rachel Dalley; Jeremy A. Miller; Thomas Chartrand; Jennie Close; Rusty Mann; Alice Mukora; Lindsay Ng; Lauren Alfiler; Katherine Baker; Darren Bertagnolli; Krissy Brouner; Tamara Casper; Eva Csajbok; Nicholas Donadio; Stan L.W. Driessens; Tom Egdorf; Rachel Enstrom; Anna A. Galakhova; Amanda Gary; Emily Gelfand; Jeff Goldy; Kristen Hadley; Tim S. Heistek; Dijon Hill; Wen-Hsien Hou; Nelson Johansen; Nik Jorstad; Lisa Kim; Agnes Katalin Kocsis; Lauren Kruse; Michael Kunst; Gabriela León; Brian Long; Matthew Mallory; Michelle Maxwell; Medea McGraw; Delissa McMillen; Erica J. Melief; Gabor Molnar; Marty T. Mortrud; Dakota Newman; Julie Nyhus; Ximena Opitz-Araya; Attila Ozsvár; Trangthanh Pham; Alice Pom; Lydia Potekhina; Ram Rajanbabu; Augustin Ruiz; Susan M. Sunkin; Ildikó Szöts; Naz Taskin; Bargavi Thyagarajan; Michael Tieu; Jessica Trinh; Sara Vargas; David Vumbaco; Femke Waleboer; Sarah Walling-Bell; Natalie Weed; Grace Williams; Julia Wilson; Shenqin Yao; Thomas Zhou; Pál Barzó; Trygve Bakken; Charles Cobbs; Nick Dee; Richard G. Ellenbogen; Luke Esposito; Manuel Ferreira; Nathan W. Gouwens; Benjamin Grannan; Ryder P. Gwinn; Jason S. Hauptman; Rebecca Hodge; Tim Jarsky; C. Dirk Keene; Andrew L. Ko; Anders Rosendal Korshoej; Boaz P. Levi; Kaare Meier; Jeffrey G. Ojemann; Anoop Patel; Jacob Ruzevick; Daniel L. Silbergeld; Kimberly Smith; Jens Christian Sørensen; Jack Waters; Hongkui Zeng; Jim Berg; Marco Capogna; Natalia A. Goriounova; Brian Kalmbach; Christiaan P.J. de Kock; Huib D. Mansvelder; Staci A. Sorensen; Gabor Tamas; Ed S. Lein; Jonathan T. Ting

doi:10.1126/SCIENCE.ADF6484

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Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex

Journal article

Peer reviewed

Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex

Brian R. Lee, Rachel Dalley, Jeremy A. Miller, Thomas Chartrand, Jennie Close, Rusty Mann, Alice Mukora, Lindsay Ng, Lauren Alfiler, Katherine Baker, …

Science, Vol.382(6667)

2023

DOI: https://doi.org/10.1126/SCIENCE.ADF6484

Abstract

Animals

Electrophysiological Phenomena

GABAergic Neurons

gamma-Aminobutyric Acid

Humans

Interneurons

Mice

Neocortex

transcription factor PAX6

4 aminobutyric acid

4 aminobutyric acid receptor

brain

cell component

neurology

RNA

Article

brain

brain tissue

brain tumor

cells by body anatomy

chemical labeling

cognition

controlled study

cytotaxonomy

electrophysiology

ex vivo study

GABAergic system

gene expression

human

human tissue

interneuron

morphology

neocortex

nerve cell

nerve cell network

neurologic disease

neurosurgery

patch clamp technique

RNA sequencing

single cell RNA seq

transcriptomics

viral genetic labeling

animal

electrophysiology

interneuron

metabolism

mouse

physiology

Human cortex transcriptomic studies have revealed a hierarchical organization of g-aminobutyric acid–producing (GABAergic) neurons from subclasses to a high diversity of more granular types. Rapid GABAergic neuron viral genetic labeling plus Patch-seq (patch-clamp electrophysiology plus single-cell RNA sequencing) sampling in human brain slices was used to reliably target and analyze GABAergic neuron subclasses and individual transcriptomic types. This characterization elucidated transitions between PVALB and SST subclasses, revealed morphological heterogeneity within an abundant transcriptomic type, identified multiple spatially distinct types of the primate-specialized double bouquet cells (DBCs), and shed light on cellular differences between homologous mouse and human neocortical GABAergic neuron types. These results highlight the importance of multimodal phenotypic characterization for refinement of emerging transcriptomic cell type taxonomies and for understanding conserved and specialized cellular properties of human brain cell types. © 2023 American Association for the Advancement of Science. All rights reserved.

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Title: Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex
Creators: Brian R. Lee - Allen Institute
Rachel Dalley - Allen Institute
Jeremy A. Miller - Allen Institute
Thomas Chartrand - Allen Institute
Jennie Close - Allen Institute
Rusty Mann - Allen Institute
Alice Mukora - Allen Institute
Lindsay Ng - Allen Institute
Lauren Alfiler - Allen Institute
Katherine Baker - Allen Institute
Darren Bertagnolli - Allen Institute for Brain Science
Krissy Brouner - Allen Institute
Tamara Casper - Allen Institute
Eva Csajbok - University of Szeged
Nicholas Donadio - Allen Institute for Brain Science
Stan L.W. Driessens - Vrije Universiteit Amsterdam
Tom Egdorf - Allen Institute
Rachel Enstrom - Allen Institute
Anna A. Galakhova - Vrije Universiteit Amsterdam
Amanda Gary - Allen Institute
Emily Gelfand - Allen Institute
Jeff Goldy - Allen Institute
Kristen Hadley - Allen Institute
Tim S. Heistek - Vrije Universiteit Amsterdam
Dijon Hill - Allen Institute
Wen-Hsien Hou
Nelson Johansen - Allen Institute
Nik Jorstad - Allen Institute
Lisa Kim - Allen Institute
Agnes Katalin Kocsis - University of Szeged
Lauren Kruse - Allen Institute
Michael Kunst - Allen Institute
Gabriela León - Allen Institute
Brian Long - Allen Institute
Matthew Mallory - Allen Institute
Michelle Maxwell - Allen Institute
Medea McGraw
Delissa McMillen - Allen Institute
Erica J. Melief - University of Washington
Gabor Molnar - University of Szeged
Marty T. Mortrud - Allen Institute
Dakota Newman - Allen Institute
Julie Nyhus - Allen Institute
Ximena Opitz-Araya - Allen Institute
Attila Ozsvár - Aarhus University
Trangthanh Pham - Allen Institute
Alice Pom
Lydia Potekhina - Allen Institute
Ram Rajanbabu - Allen Institute
Augustin Ruiz - Allen Institute
Susan M. Sunkin - Allen Institute for Brain Science
Ildikó Szöts - University of Szeged
Naz Taskin - Allen Institute for Brain Science
Bargavi Thyagarajan - Allen Institute
Michael Tieu - Allen Institute
Jessica Trinh - Allen Institute
Sara Vargas - Allen Institute
David Vumbaco - Allen Institute
Femke Waleboer - Vrije Universiteit Amsterdam
Sarah Walling-Bell - Allen Institute
Natalie Weed - Allen Institute
Grace Williams - Allen Institute
Julia Wilson - Allen Institute for Brain Science
Shenqin Yao - Allen Institute
Thomas Zhou - Allen Institute
Pál Barzó - University of Szeged
Trygve Bakken - Allen Institute for Brain Science
Charles Cobbs - Swedish Medical Center
Nick Dee - Allen Institute
Richard G. Ellenbogen - Neurological Surgery
Luke Esposito - Allen Institute
Manuel Ferreira - Neurological Surgery
Nathan W. Gouwens - Allen Institute for Brain Science
Benjamin Grannan - Neurological Surgery
Ryder P. Gwinn - Swedish Medical Center
Jason S. Hauptman - Neurological Surgery
Rebecca Hodge - Allen Institute
Tim Jarsky - Allen Institute
C. Dirk Keene - University of Washington
Andrew L. Ko - Neurological Surgery
Anders Rosendal Korshoej - Aarhus University Hospital
Boaz P. Levi - Allen Institute
Kaare Meier - Aarhus University Hospital
Jeffrey G. Ojemann - Neurological Surgery
Anoop Patel - University of Washington
Jacob Ruzevick - Neurological Surgery
Daniel L. Silbergeld - Neurological Surgery
Kimberly Smith - Allen Institute
Jens Christian Sørensen
Jack Waters - Allen Institute
Hongkui Zeng - Allen Institute
Jim Berg - Allen Institute
Marco Capogna - Aarhus University
Natalia A. Goriounova - Vrije Universiteit Amsterdam
Brian Kalmbach - Allen Institute for Brain Science
Christiaan P.J. de Kock - Vrije Universiteit Amsterdam
Huib D. Mansvelder
Staci A. Sorensen - Allen Institute for Brain Science
Gabor Tamas - University of Szeged
Ed S. Lein - Allen Institute
Jonathan T. Ting - Allen Institute
Publication Details: Science, Vol.382(6667)
Publisher: American Association for the Advancement of Science
Identifiers: 991006161399802656
Language: English
Resource Type: Journal article

Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex

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